Journal: Journal of Neuroinflammation

Article Title: The activation of hippocampus microglia in the occurrence of airway hyperresponsiveness comorbidity anxiety

doi: 10.1186/s12974-025-03565-6

Figure Lengend Snippet: Airway hyperresponsiveness in both OVA and CRS mouse models. A , B Schematic illustration of the protocol for AHR model and anxiety model establishment ( n = 8). C Airway resistance of mice in response to nebulized methacholine of different concentrations (0, 6, 12, 24 and 48 mg/ml; n = 4). D Commercial ELISA kits were used to assess the concentration of IL-4, IL-5 and IL-13 in bronchoalveolar lavage fluid ( n = 3), one-way ANOVA. Data were presented as mean ± SEM. * P < 0.05, ** P < 0.01, and *** P < 0.001

Article Snippet: Commercial ELISA kits (IL-4, cat. no. EK0405; IL-5, cat. no. EK0408; and IL-13, cat. no. EK0425; all from Boster Biological Technology) were used to detect the levels of IL-4, IL-5 and IL-13 in BALF according to the manufacturer’s protocol.

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay